Crossbreeding effect of double-muscled cattle on in vitro embryo development and quality |
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| Affiliation: | 1. Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium;2. Department of Theriogenology and Animal Production, Usmanu Danfodiyo University, Sokoto, Nigeria;3. Department of Veterinary Sciences, Gamete Research Center, Veterinary Physiology and Biochemistry, University of Antwerp, Wilrijk, Belgium;1. Eutheria Foundation, Cross Plains, WI, 53528, USA;2. Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, 53706, USA;3. Departments of Animal Sciences and Dairy Science. University of Wisconsin-Madison, Madison, WI, 53706, USA;1. Faculty of Medicine, Universiti Teknologi MARA Sungai Buloh Campus, Selangor, Malaysia;2. I-PPerFORM, Universiti Teknologi MARA, Malaysia;1. Department of Stem Cells and Regenerative Medicine, Institute of Natural Fibers and Medicinal Plants, Poznan, Poland;2. Department of General Pharmacology and Pharmacoeconomics, Pomeranian Medical University, Szczecin, Poland;3. Division of Perinatology and Women’s Diseases, Poznan University of Medical Sciences, Poznan, Poland;4. Clinic of Operational Gynecology, Poznan University of Medical Sciences, Poznan, Poland;5. Department of Pharmacology and Phytochemistry, Institute of Natural Fibers and Medicinal Plants, Poznan, Poland;6. Clinic of Pediatrics, Endocrinology, Diabetology, Metabolic Diseases and Cardiology of the Developmental Age, Pomeranian Medical University, Szczecin, Poland;7. Department of Histocompatibility with Laboratory of Genetic Diagnostics, Regional Blood Center, Poznan, Poland;1. Unidad Académica de Ciencias Agropecuarias, Universidad Católica de Cuenca, Ecuador;2. Laboratorio de Biotecnologías de la Reproducción Animal, Facultad de Ciencias Agropecuarias. Universidad de Cuenca, Ecuador;3. Facultad de Ciencias Veterinarias, Universidad del Zulia, Venezuela;4. Departamento de Ciencias Agrarias, Universidad de Los Andes, Trujillo, Venezuela;1. PIVET Medical Centre, Perth 6007, WA, Australia;2. School of Pharmacy and Biomedical Science, Faculty of Health Sciences, Curtin University, Perth 6845, WA, Australia;3. Hospital Tengku Ampuan Rahimah (HTAR), Klang, Malaysia;4. School of Public Health, Faculty of Health Sciences, Curtin University, Perth 6845, WA, Australia;1. Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China;2. Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China;3. Department of Veterinary Anatomy & Histology, Shaheed Benazir Bhutto University of Veterinary and Animal Sciences, Sakrand, 67210, Sindh, Pakistan;4. Department of Veterinary Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China;5. Department of Veterinary Parasitology, Faculty of Veterinary Sciences, Shaheed Benazir Bhutto University of Veterinary and Animal Sciences, Sakrand, 67210, Sindh, Pakistan |
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| Abstract: | Nowadays, several developing countries have started to breed double-muscled cattle to their autochthonous cattle to improve meat production. However, the developmental competence of the resultant crossbreeding embryos is unknown. The objective of this study was to evaluate the effect of crossbreeding double-muscled (Belgian Blue; BB) semen with beef (Limousin; LIM) and dairy (Holstein-Friesian; HF) derived oocytes on embryo development and quality, using purebred BB as a control (BB oocytes fertilized by BB sperm). A single ejaculate of a BB bull was evaluated by Computer Assisted Sperm Analysis before using for in vitro fertilization. Ovaries from each breed were collected at the local slaughterhouse (n = 1,720 oocytes). All statistical analyses were performed using R-core (P < 0.05). Embryo quality was evaluated via differential-apoptotic staining of day 8 blastocysts. Cleavage (48 h post insemination) and day 8 blastocyst rates were greater (P < 0.05) for LIM (82.9 ± 6 and 27 ± 4.3%, respectively) than for BB (69.8 ± 8.5 and 19.6 ± 3.1%, respectively) and HF (45.1 ± 10 and 12.3 ± 2.2%, respectively). Holstein-Friesian presented lower cleavage and day 8 blastocyst rates than BB (P < 0.05). Limousin blastocysts presented a higher number (P < 0.05) of inner cell mass cells (ICM; 68 ± 7.8) than HF (40.4 ± 8.2). In conclusion, crossbreeding double-muscled cattle by in vitro fertilization with LIM oocytes yielded better embryo compared with the purebred combination, while the combination with HF oocytes produced the lowest rate of blastocysts. |
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| Keywords: | fertility semen oocyte |
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