Monitoring the rate of oxygen consumption in plant cell suspensions |
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Authors: | Baker C Jacyn Mock Norton M Deahl Kenneth Domek John |
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Institution: | (1) Department of Agriculture, Microbiology and Plant Pathology Laboratory and Vegetable Laboratory, ARS, United States, Beltsville, MD, 20705, U.S.A;(2) Department of Agriculture, ARS, United States, Beltsville, MD, 20705, U.S.A |
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Abstract: | A method is described that allows the rate of oxygen consumption to be monitored in plant cell suspensions. The method utilized
oxygen electrodes placed in beakers of plant cells subjected to various treatments. The voltage readings from calibrated electrodes
were converted to % oxygen (100% equals air equilibration) and the rate of oxygen consumption was estimated by calibration
graphs made with no cells present. This system simultaneously monitors one to sixteen or more samples, allowing comparison
of treatments on identically treated cells. We have used this method to study the respiratory burst of plant cells produced
in response to viable or heat-killed bacteria. Because the system was computer-monitored and open to the atmosphere, data
could be collected over several hours. Various factors that affected the measurement of dissolved oxygen concentration with
this technique were explored and considered.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | Nicotiana tabacum oxidative burst oxygen electrode Pseudomonas solanacearum Pseudomonas syringae respiratory burst Solanumtuberosum |
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