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| 蛹虫草表型多态性对子实体产生及虫草菌素的影响 | |
| 引用本文： | 李光荣,文庭池,康冀川,康超,雷帮星. 蛹虫草表型多态性对子实体产生及虫草菌素的影响[J]. 微生物学通报, 2011, 38(3): 370-382 |
| 作者姓名： | 李光荣 文庭池 康冀川 康超 雷帮星 |
| 作者单位： | 1. 西南药用生物资源教育部工程研究中心,贵州贵阳,550025;贵州大学贵州省农业生物工程重点实验室,贵州贵阳,550025;贵州大学生命科学学院,贵州贵阳,550025 2. 西南药用生物资源教育部工程研究中心,贵州贵阳,550025 3. 西南药用生物资源教育部工程研究中心,贵州贵阳,550025;贵州大学生命科学学院,贵州贵阳,550025 |
| 基金项目： | 贵州省科学技术基金项目(No. 黔科合J 字[2008]2089); 贵州省教育厅自然科学研究项目[No. 黔教科(2009) 0129 号]; 贵州大学研究生创新基金项目(No. 校研农2009018) |
| 摘 要： | 为选育高产子实体和虫草菌素的蛹虫草菌株,从野生蛹虫草中分离单孢子并进行分型,之后进行产子实体实验;同时对蛹虫草子实体进行了产孢结构的观察,并用高效液相色谱仪检测了子实体和培养基中的虫草菌素。结果表明,菌落背面颜色为橙铬色的菌株容易产生子实体;出发(原代)菌株所产子实体在形态上更接近野生蛹虫草,而角变株的子实体畸形;出发菌株产子实体能力要比该菌株未发生角变部分分离株和角变部分分离株都强。在虫草菌素的产量上也以出发菌株为高,表明表型多态性对蛹虫草产子实体和虫草菌素有很大的影响。这对于优势菌种的筛选和生产实践有借鉴意义。 |
| 关 键 词： | 蛹虫草 表型多态性 子实体 虫草菌素 |
| 收稿时间： | 2010-09-02 |
| 修稿时间： | 2010-11-29 |
The effect of polymorphism on fruiting body and cordycepin production of Cordyceps militaris |
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| LI Guang-Rong,WEN Ting-Chi,KANG Ji-Chuan,KANG Chao and LEI Bang-Xing. The effect of polymorphism on fruiting body and cordycepin production of Cordyceps militaris[J]. Microbiology China, 2011, 38(3): 370-382 | |
| Authors: | LI Guang-Rong WEN Ting-Chi KANG Ji-Chuan KANG Chao LEI Bang-Xing |
| Affiliation: | The Engineering and Research Center for Southwest Bio-Pharmaceutical Resources of National Education Ministry of China, Guiyang, Guizhou 550025, China; Guizhou Key Laboratory of Agricultural Bioengineering, Guizhou University, Guiyang, Guizhou 550025, Chi;The Engineering and Research Center for Southwest Bio-Pharmaceutical Resources of National Education Ministry of China, Guiyang, Guizhou 550025, China;The Engineering and Research Center for Southwest Bio-Pharmaceutical Resources of National Education Ministry of China, Guiyang, Guizhou 550025, China; Guizhou Key Laboratory of Agricultural Bioengineering, Guizhou University, Guiyang, Guizhou 550025, Chi;The Engineering and Research Center for Southwest Bio-Pharmaceutical Resources of National Education Ministry of China, Guiyang, Guizhou 550025, China; College of Life Sciences, Guizhou University, Guiyang, Guizhou 550025, China;The Engineering and Research Center for Southwest Bio-Pharmaceutical Resources of National Education Ministry of China, Guiyang, Guizhou 550025, China |
| Abstract: | In order to screen and breed the strain with high fruiting body and cordycepin production, this study aims to investigate the effect of polymorphism on fruiting body and cordycepin production of C. militaris. Three progeny population were isolated from wild fruitbody of C. militaris, cultural characteristics of ascospores and fruitbody-producing ability were observed, and determined Cordycepin content in fruitbody and medium by HPLC. The results showed that the strains with colony reverse color of orange chrome could produce fruiting body easily. The sector mutation proportion of the strain produce few or no fruiting body. The shape and microstructure of fruiting body produced by original strain were similar to the wild strain in the nature. The original strain produced more fruiting body and cordycepin than the strains isolated from sector mutation proportion and the other parts without sector mutation. These results demonstrated the effect of polymorphism on fruiting body and cordycepin production of C. militaris. |
| Keywords: | Cordyceps militaris Polymorphism Fruiting body Cordycepin |
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