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Pipecolic acid pathway: the major lysine metabolic route in the rat brain.
Authors:Y F Chang
Institution:1. Department of Obstetrics and Gynecology, College of Physicians & Surgeons, Columbia University, New York, New York 10032 USA.;2. Department of Medicine, College of Physicians & Surgeons, Columbia University, New York, New York 10032 USA.
Abstract:A peptide of 6,700–8,000 daltons has been isolated from an ethanolic KOH digest of bovine ligamentum nuchae elastin by a combination of ion exchange chromatography and gel filtration techniques. The peptide is non-polar and is composed almost entirely of glycine, valine, and proline (or hydroxyproline) in the ratio 2:2:1. Edman degradation of the first 17 residues gave the sequence: NH2-Gly-Phe-Pro-Gly-Val-Gly-Val-Pro-Gly-Val-Gly-Val-Pro-Gly-Val-Gly-Val. These results suggest that the peptide is made up of repeating pentapeptide units with the sequence: Pro-Gly-Val-Gly-Val.
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