Effects of ovariectomy on microsatellite instability in rat colon tumors induced by 1,2-dimethylhydrazine |
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Authors: | Fei Xu Guobin Wang Kailin Cai Ronglin Zhai Shouyuan Tang |
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Institution: | (1) Department of General Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 Jiefang Avenue, 430022 Wuhan, Hubei Province, China; |
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Abstract: | To study the effects of ovariectomy on tumorigenesis and microsatellite instability (MSI) in rat colon tumors induced by 1,2-dimethylhydrazine,
to elucidate the association between postmenopausal ovarian hormones depletion and MSI pathway in colorectal tumorigenesis.
Forty female Wistar rats were randomly divided into two groups: Ovariectomized (Ovx) group and Sham-ovariectomized (Sham-Ovx)
group. All rats were injected intraperitoneally with 1,2-dimethylhydrazine (DMH) (20 mg/kg b.w) once a week for 20 weeks.
Ten weeks after the final DMH injection, all the rats were sacrificed to collect tumors. Microsatellite instability of six
microsatellite loci was detected using fluorescent PCR followed by fragment analysis on automatic DNA sequencer with GeneScan
3.7 software. The tumor multiplicity in the OVX group was significantly higher than that in the Sham-OVX group (3.6 ± 1.4
vs. 2.4 ± 1.6, P < 0.05). The incidence of MSI-positive tumors in OVX group was higher than that in Sham-OVX group (32.1 vs. 10.8%, P < 0.05).The incidence of tumors showing MSI at multiple loci in OVX group was also higher than that in Sham-OVX group (18.9
vs. 2.7%, P < 0.05). Ovariectomy increased tumor formation and the frequency of MSI in DMH-induced colon tumors. It implied that postmenopausal
ovarian hormones depletion might influence colorectal tumorigenesis through MSI pathway. |
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Keywords: | |
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