Inorganic polyphosphate in the yeast <Emphasis Type="Italic">Saccharomyces cerevisiae</Emphasis> with a mutation disturbing the function of vacuolar ATPase |
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Authors: | A A Tomaschevsky L P Ryasanova T V Kulakovskaya I S Kulaev |
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Institution: | Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia. |
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Abstract: | A mutation in the vma2 gene disturbing V-ATPase function in the yeast Saccharomyces cerevisiae results in a five- and threefold decrease in inorganic polyphosphate content in the stationary and active phases of growth
on glucose, respectively. The average polyphosphate chain length in the mutant cells is decreased. The mutation does not prevent
polyphosphate utilization during cultivation in a phosphate-deficient medium and recovery of its level on reinoculation in
complete medium after phosphate deficiency. The content of short chain acid-soluble polyphosphates is recovered first. It
is supposed that these polyphosphates are less dependent on the electrochemical gradient on the vacuolar membrane. |
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