Incomplete concerted evolution and reproductive isolation at the rDNA locus uncovers nine cryptic species within Anopheles longirostrisfrom Papua New Guinea |
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| Authors: | David E Alquezar Stephane Hemmerter Robert D Cooper Nigel W Beebe |
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| Affiliation: | 1.Institute for the Biotechnology of Infectious Disease,University of Technology,Sydney,Australia;2.Australian Army Malaria Institute,Brisbane,Australia;3.School of Biological Sciences,University of Queensland,St Lucia,Australia;4.CSIRO Ecosystem Sciences,EcoSciences Precinct,Dutton Park,Australia |
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| Abstract: | Background Nuclear ribosomal DNA (rDNA) genes and transcribed spacers are highly utilized as taxonomic markers in metazoans despite the lack of a cohesive understanding of their evolution. Here we follow the evolution of the rDNA second internal transcribed spacer (ITS2) and the mitochondrial DNA cytochrome oxidase I subunit in the malaria mosquito Anopheles longirostris from Papua New Guinea (PNG). This morphospecies inhabits a variety of ecological environments indicating that it may comprise a complex of morphologically indistinguishable species. Using collections from over 70 sites in PNG, the mtDNA was assessed via direct DNA sequencing while the ITS2 was assessed at three levels - crude sequence variation through restriction digest, intragenomic copy variant organisation (homogenisation) through heteroduplex analysis and DNA sequencing via cloning. |
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