Distribution of hydroxyindole-O-methyltransferase mRNA in the rat brain: an in situ hybridisation study |
| |
Authors: | Christophe Ribelayga François Gauer Paul Pévet Valérie Simonneaux |
| |
Institution: | (1) Neurobiologie des Fonctions Rythmiques et Saisonnières, UMR-CNRS 7518, Université Louis Pasteur, 12 Rue de l’Université, F-67000 Strasbourg, France Tel.: +33-3-88-358504, Fax: +33-3-88-240461, e-mail: simonneaux@neurochem.u-strasbg.fr, FR |
| |
Abstract: | Hydroxyindole-O-methyltransferase (HIOMT) is the enzyme involved in the last step of the melatonin synthesis pathway. Recently,
a cDNA encoding HIOMT has been isolated from a rat pineal gland library. Using this cDNA, we developed a highly sensitive
in situ hybridisation protocol to investigate the distribution of HIOMT mRNA in both the rat brain and dissociated pinealocytes
maintained in primary cell culture. In the rat brain, HIOMT mRNA was only detected in the three parts of the pineal complex:
the superficial pineal, the stalk and the deep pineal. No extra-pineal hybridisation labelling was observed. These results
strongly suggest that melatonin synthesis also occurs in the deep part and the stalk of the pineal gland. HIOMT mRNA was markedly
expressed in cultured pinealocytes. No particular subcellular area was observed to express HIOMT mRNA specifically, as the
labelling was homogeneously distributed in the cytosol and in the axon-like processes. In conclusion, the use of in situ and
in vitro hybridisation with a pineal riboprobe has detected notable HIOMT expression restricted to pinealocytes.
Received: 26 June 1997 / Accepted: 15 September 1997 |
| |
Keywords: | Hydroxyindole-O-methyltransferase Pineal gland In situ hybridisation Melatonin 5-Methoxyindoles Rat (Wistar) |
本文献已被 SpringerLink 等数据库收录! |
|