Purification and characterization of a β-glucosidase from the phytopathogenic fungus Fusarium oxysporum f. sp. melonis |
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| Authors: | TM Alconada MJ Martínez |
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| Institution: | Departamento de Cs. Biológicas, Facultad de Cs. Exactas y Naturales, Ciudad Universitaria, Buenos Aires, Argentina;Centro de Investigaciones Biológicas, CSIC, Departamento de Microbiología Molecular, Madrid, Spain |
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| Abstract: | T.M. ALCONADA AND M.J. MARTÍNEZ. 1996. Fusarium oxysporum f. sp. melonis produces cellulase and β-glucosidase activities in a medium with glucose and avicel as carbon source. A β-glucosidase from this crude material was purified by gel filtration and ion exchange chromatography successively. This enzyme is a unique band of protein in SDS-PAGE and isoelectric focussing. It had a molecular weight of 66000 and a pI of 5. Using p -nitrophenyl-β-D-glucopyranoside as substrate β-glucosidase shows a K m of 210 μmol 1-1, an optimum pH of 5.5 and an optimum reaction temperature of 60°C, being stable in a pH range of 5–7 for 48 h at room temperature. |
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