Altered structure of HLA class I heavy chains associated with mouse beta-2 microglobulin |
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Authors: | Pierre Ferrie Juan C Fontecilla-Camps Danielle Bucchini Danièle H Caillol Bertrand R Jordan François A Lemonnier |
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Institution: | (1) Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, Case 906, 13288 Marseille cédex 09, France;(2) Centre de Recherche sur les Mécanismes de la Croissance Cristalline, Case 913, 13288 Marseille cédex 09, France;(3) INSERM U. 257, Institut Jacques Monod du CNRS et de l'Université Paris VII, 75251 Paris cédex 05, France |
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Abstract: | The serological reactivities of HLA-A3, -B7, and -CW3 heavy chains associated with either mouse, bovine, or human beta-2 microglobulin (
2m) and expressed on the surface of transfected mouse fibroblasts were analyzed. All reactivities associated with one cluster (defined by monoclonal antibody W6/32) of antigenic determinants expressed by these HLA class I molecules were lost, or profoundly reduced, after each heavy chain associated with mouse
2-m. Expression by the transfected fibroblasts of the HLA-A3, -B7, and -CW3 heavy chains in association with human
2m restores these reactivities. Since most of the amino acid differences between mouse and human
2m probably correspond to externally oriented hydrophilic residues, these results suggest that critical interactions in the three-dimensional structure of HLA class I molecules occur between the light chain and the first two external domains of the class I heavy chains, to which some of the altered reactivities have been mapped. |
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