Femtomolar DNA detection by parallel colorimetric darkfield microscopy of functionalized gold nanoparticles |
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Authors: | Verdoold Remco Gill Ron Ungureanu Felicia Molenaar Robert Kooyman Rob P H |
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Affiliation: | MESA+ Institute for Nanotechnology, University of Twente, AE Enschede, The Netherlands. r.verdoold@utwente.nl |
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Abstract: | We introduce a sensing platform for specific detection of DNA based on the formation of gold nanoparticles dimers on a surface. The specific coupling of a second gold nanoparticle to a surface bound nanoparticle by DNA hybridization results in a red shift of the nanoparticle plasmon peak. This shift can be detected as a color change in the darkfield image of the gold nanoparticles. Parallel detection of hundreds of gold nanoparticles with a calibrated true color camera enabled us to detect specific binding of target DNA. This enables a limit of detection below 1.0×10(-14) M without the need for a spectrometer or a scanning stage. |
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