Transformation of Escherichia coli mediated by natural phospholipids |
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| Authors: | Sato Yuko Kumazawa Noriyuki Yoshikawa Kenichi Kurusu Yasurou |
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| Institution: | Laboratory of Molecular Microbiology, College of Agriculture, Ibaraki University, Ibaraki, Japan. |
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| Abstract: | Transformation system for Escherichia coli based upon introduction of plasmid DNA by natural phospholipids has been developed. Transformants are easily obtained by treatment with natural phospholipids such as phosphatidylethanolamine, phosphatidylcoline, and phosphatidylserine, where the presence of MgCl2 or CaCl2 is essential. This method of transformation is applicable not only for small plasmid pHSG399 (2.3 Kb) but also for giant plasmid R6K (100 Kb). |
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