Lysis protein S of phage lambda functions in Saccharomyces cerevisiae. |
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Authors: | J Garrett C Bruno R Young |
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Affiliation: | Department of Biology, Hamilton College, Clinton, New York 13323. |
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Abstract: | The lambda S lysis gene was cloned into a Saccharomyces cerevisiae expression vector under GAL1 control. Induction with galactose in S. cerevisiae terminated cell growth and prevented colony formation. Several membrane proteins immunoreactive with anti-S antibody accumulated in the membranes, indicating that sodium dodecyl sulfate-resistant oligomers of S are formed, similar to those observed in the membranes of Escherichia coli cells killed by expression of the S gene. These observations suggest that the S gene product functions as a cytotoxic protein in the yeast cytoplasmic membrane as it does in the bacterial membrane. |
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