Intramolecularly quenched fluorogenic tetrapeptide substrates for tissue and plasma kallikreins |
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Authors: | J R Chagas L Juliano E S Prado |
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Institution: | Department of Biophysics, Escola Paulista de Medicina, Sao Paulo, Brazil. |
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Abstract: | Five intramolecularly quenched fluorogenic substrates for arginyl hydrolases with the sequence Abz-Phe-Arg-X-Y-EDDnp (X = Arg or Ser; Y = Val, Pro, or Arg) were synthesized by classical solution methods. Kinetics of their hydrolysis by tissue and plasma kallikreins, trypsin, and thrombin characterized Abz-Phe-Arg-Ser-Arg-EDDnp as a specific and sensitive substrate for the continuous assay of tissue kallikreins while Abz-Phe-Arg-Arg-Pro-EDDnp was the best substrate for human plasma kallikrein. The five peptides were poor substrates for trypsin and resistant to thrombin. |
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