Rapid Removal of Acid from Glycol Methacrylate for Improved Histological Embedding |
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| Authors: | Robin Frater |
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| Institution: |
a Division of Protein Chemistry, Commonwealth Scientific and Industrial Research Organization, Melbourne, Australia |
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| Abstract: | A method for the rapid and complete removal of methacrylic acid from commercial samples of glycol methacrylate is presented. It entails conversion of the acid to an insoluble N-acylurea by treatment with an equivalent amount of N, N'-dicyclohexylcarbodiimide. Sections of tissue embedded in polymer prepared from the purified monomer can be stained with basic dyes without simultaneously staining the polymer. |
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