Chain conformation of carboxymethylated derivatives of (1 → 3)-β-d-glucan from Poria cocos sclerotium |
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Authors: | Yifeng Wang Lina Zhang |
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Institution: | aDepartment of Chemistry, Wuhan University, Wuhan 430072, China |
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Abstract: | A water-insoluble (1 → 3)-β-d-glucan (PCSG) isolated from the fresh sclerotium of Poria cocos was carboxymethylated to afford a water-soluble derivative coded as C-PCSG. The carboxymethylated (1 → 3)-β-d-glucan was fractionated to obtain eight fractions according to the nonsolvent addition method. The weight-average molecular mass (Mw), radius of gyration and intrinsic viscosity (η]) of the fractions were determined by size-exclusion chromatography combined with laser light scattering (SEC-LLS) and viscometry in 0.2 M NaCl aqueous solution at 25 °C. The dependences of η] and on Mw for C-PCSG were found to be , and (nm), respectively. Analysis of Mw and η] in terms of the known theories for wormlike chain model yielded 633 nm−1 for molar mass per unit contour length (ML), 5.5 nm for persistence length (q), and 20.2 for characteristic ratio (C∞). These results indicated that C-PCSG exists as a relatively extended flexible chain in 0.2 M NaCl aqueous solution. Therefore, the introduction of the carboxymethyl groups into the β-glucan improved significantly the water solubility and enhanced the stiffness of the chains. |
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Keywords: | (1  →   3)-β d-Glucan" target="_blank">-d-Glucan Carboxymethylated derivative Poria cocos sclerotium Solution properties Chain conformation |
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