Plant defense in response to chewing insects: proteome analysis of Arabidopsis thaliana damaged by Plutella xylostella |
| |
Authors: | Dieu-Hien Truong Hoang Chinh Nguyen Julien Bauwens Gabriel Mazzucchelli Georges Lognay Frédéric Francis |
| |
Institution: | 1. Faculty of Applied Sciences, Ton Duc Thang University, Ho Chi Minh city, Vietnam;2. Functional &3. Evolutionary Entomology, University of Liège, Gembloux Agro-Bio Tech, Wallonia, Belgium;4. Mass Spectrometry Laboratory, University of Liège, Liège, Belgium;5. Analytical Chemistry Laboratory, University of Liège, Gembloux Agro-Bio Tech, Wallonia, Belgium |
| |
Abstract: | The interactions between Arabidopsis thaliana and Plutella xylostella have been considered as a model system to unravel the responses of plants to herbivorous insects. Here, we use a 2-DE proteome approach to detect protein expression changes in the leaves of Arabidopsis plants exposed to P. xylostella larval infestation at 27°C within 8?h. Approximately 450 protein spots were reproducibly detected on gels. Of these, comparing healthy and infested leaves, we identified 18 differentially expressed protein spots. Thirteen proteins were successfully identified by MALDI-TOF/MS and LC-ESI-MS/MS. Functional classification analysis indicated that the differentially identified proteins were associated with amino acid, carbohydrate, energy, lipid metabolism, and photosynthesis. In addition, their relative abundances were assessed according to larval pest feeding on Arabidopsis leaves. These data provide valuable new insights for further works in plant-biotic and environmental stress interaction. |
| |
Keywords: | Arabidopsis thaliana Plutella xylostella proteomic expression 2-DE MALDI-TOF MS LC-ESI-MS/MS |
|
|