| The effect of ethylene inhibitors （AgNO3, AVG）, an ethyleneliberating compound （CEPA） and aeration on the formation of protocorm-like bodies of hybrid Cymbidium （Orchidaceae） |
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| 引用本文： | Jaime A. TEIXEIRA DA SILVA. The effect of ethylene inhibitors （AgNO3, AVG）, an ethyleneliberating compound （CEPA） and aeration on the formation of protocorm-like bodies of hybrid Cymbidium （Orchidaceae）[J]. 生物学前沿, 2013, 8(6): 606-610. DOI: 10.1007/s11515-013-1283-x |
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| 作者姓名： | Jaime A. TEIXEIRA DA SILVA |
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| 作者单位： | [1]Faculty of Agriculture and Graduate School of Agriculture, Kagawa University, Miki-cho, Kagawa, 761-0795, Japan [2]Present address. P O. Box 7, Miki-cho post office, Ikenobe 3011-2, Kagawa-ken, 761-0799, Japan |
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| 基金项目： | The author thanks Prof. Michio Tanaka for research support. |
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| 摘 要： | Protocorm-like bodies （PLBs） or thin cell layers （TCLs） derived from PLBs of hybrid Cymbidium Twilight Moon ＇Day Light＇ can induce new or neo-PLBs on Teixeira Cymbidium （TC） medium, which contains 0.1 mg/L a- naphthaleneacetic acid, 0.1 mg/L kinetin, 2 g/L tryptone and 20 g/L sucrose, and is solidified with 8 g/L Bacto agar. This study aimed to assess the response ofneo-PLB formation to an ethylene-liberating compound （2-chloroethylphosphonic acid （CEPA））, to two ethylene inhibitors （silver nitrate （AgNO3） and aminoethoxyvinylglycine （AVG））, and to aeration （made possible by using Milliseal~ or autoclaved filter paper）. AgNO3 at 1 or 2 mg/L in TC medium significantly increased the fresh weight of PLBs while 1 mg/L of AgNO3 also showed a significant increase in the number ofneo-PLB from both half-PLBs and from tTCLs. In contrast, AVG and CEPA inhibited neo-PLB formation. Neo-PLB formation from half-PLB or TCL explants in the presence of aeration resulted in significantly lower neo-PLB weight. The use of AgNO3 and aeration are alternative means to mass produce neo-PLBs for micropropagation purposes.
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| 关 键 词： | 乙烯抑制剂 原球茎状体 大花蕙兰 CEPA 硝酸银 AVG 化合物 曝气 |
The effect of ethylene inhibitors (AgNO3, AVG), an ethylene-liberating compound (CEPA) and aeration on the formation of protocorm-like bodies of hybrid Cymbidium (Orchidaceae) |
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| Jaime A. Teixeira Da Silva. The effect of ethylene inhibitors (AgNO3, AVG), an ethylene-liberating compound (CEPA) and aeration on the formation of protocorm-like bodies of hybrid Cymbidium (Orchidaceae)[J]. Frontiers in Biology, 2013, 8(6): 606-610. DOI: 10.1007/s11515-013-1283-x |
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| Authors: | Jaime A. Teixeira Da Silva |
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| Affiliation: | 1. Faculty of Agriculture and Graduate School of Agriculture, Kagawa University, Miki-cho, Kagawa, 761-0795, Japan
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| Abstract: | Protocorm-like bodies (PLBs) or thin cell layers (TCLs) derived from PLBs of hybrid Cymbidium Twilight Moon ‘Day Light’ can induce new or neo-PLBs on Teixeira Cymbidium (TC) medium, which contains 0.1 mg/L α-naphthaleneacetic acid, 0.1 mg/L kinetin, 2 g/L tryptone and 20 g/L sucrose, and is solidified with 8 g/L Bacto agar. This study aimed to assess the response of neo-PLB formation to an ethylene-liberating compound (2-chloroethylphosphonic acid (CEPA)), to two ethylene inhibitors (silver nitrate (AgNO3) and aminoethoxyvinylglycine (AVG)), and to aeration (made possible by using Milliseal® or autoclaved filter paper). AgNO3 at 1 or 2 mg/L in TC medium significantly increased the fresh weight of PLBs while 1 mg/L of AgNO3 also showed a significant increase in the number of neo-PLB from both half-PLBs and from tTCLs. In contrast, AVG and CEPA inhibited neo-PLB formation. Neo-PLB formation from half-PLB or TCL explants in the presence of aeration resulted in significantly lower neo-PLB weight. The use of AgNO3 and aeration are alternative means to mass produce neo-PLBs for micropropagation purposes. |
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| Keywords: | AgNO3 AVG CEPA PLB Teixeira Cymbidium （TC） medium |
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