Restriction of anti-peptide antibody specificity by enzyme-modified Sepharose-peptide immunoadsorbents |
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Authors: | M L Trinca E Muratti M Camera A Chersi |
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Institution: | Istituto Regina Elena for Cancer Research, Lab. of Biochemistry, Rome, Italy. |
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Abstract: | Sepharose-peptide immunoadsorbents, employed for the isolation of specific antibodies from the sera of rabbits immunized with carrier protein-peptide conjugates, were digested with suitable proteolytic enzymes, in order to obtain the splitting of a part of the peptide bound to the gel. This new modified immunoadsorbent can be advantageously used for the isolation of antibody subsets, that do not cross-react with related peptides exhibiting high sequence homology with the immunogens. |
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