Staining Etched Epoxy Resin Sections for Light Microscopy |
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Authors: | Tsukasa Iwadare Taizo Arai |
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Institution: |
a Research Laboratory, Sakura Finetechnical Co., Tokyo, Japan
b Department of Pathology, Tokyo Teishin Hospital, Tokyo, Japan |
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Abstract: | Staining of etched sections for light microscopy is described. Azan staining was successful after treatment with potassium dichromate and the use of concentrated dye solutions. To remove osmium for hematoxylin-eosin staining, removal by reduction with ferrocene was used instead of oxidation. Highly selective differentiation after hematoxylin staining was achieved using p-toluenesulfonic acid-DMSO. To enhance eosin staining, a 2-bromoethylamine link between eosin and the tissue was used. Ferrocene also facilitated counterstaining of nuclei with hematoxylin after the PAS reaction. Periodic acid-methenamine silver staining was carried out without modification. |
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Keywords: | azan 2-bromoethylamine epoxy section ferrocene hematoxylin-eosin kidney biopsy light microscopy PAS periodic acid-methanamine silver osmium removal etched section p-toluenesulfonic acid DMSO |
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