A small heat shock protein of Ostertagia ostertagi: stage-specific expression, heat inducibility, and protection trial |
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| Authors: | Vercauteren Isabel De Maere Veerle Vercruysse Jozef Stevens Mieke Gevaert Kris Claerebout Edwin |
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| Institution: | Laboratory of Parasitology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan, 133, B-9820 Merelbeke, Belgium. |
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| Abstract: | In this study, we isolated and analyzed a small heat shock protein (HSP) of Ostertagia ostertagi (Oo-HSP18). Oo-hsp18 is encoded by a single-copy gene and the full-length cDNA represents an 18-kDa protein. The expression of Oo-hsp18 is highly stage specific and restricted to the adult stage. The protein is synthesized in a tissue-specific manner and localized in the body muscle layer. The levels of Oo-hsp18 mRNAs are sharply induced by heat shock but not by other stressors such as levamisole and H2O2. A vaccination trial with recombinant Oo-HSP18 failed to protect calves against a challenge infection. |
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