| 超氧化物歧化酶和Bcl-2在实验性精索静脉曲张大鼠睾丸中的变化 |
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| 引用本文： | 贾月峰,王新生,马征,王沛涛. 超氧化物歧化酶和Bcl-2在实验性精索静脉曲张大鼠睾丸中的变化[J]. 现代生物医学进展, 2010, 10(4) |
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| 作者姓名： | 贾月峰 王新生 马征 王沛涛 |
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| 作者单位： | 青岛大学医学院附属医院,山东,青岛,266021 |
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| 摘 要： | 目的:建立大鼠实验性精索静脉曲张(experimental varicocele EV)的模式,测量睾丸中超氧化物歧化酶(superoxide dismutaseSOD)活性和Bcl-2的表达。方法:将40只雄性青春期Wistar大鼠随机分为EV8周组和12周组(各12只)和相应的假手术对照组2组(各8只),通过部分结扎左肾静脉建立大鼠EV模型,分别于术后8周、12周处死动物,测左侧精索静脉直径,用比色法测SOD 活力,免疫组化法测Bcl-2的表达。结果:成功建立了EV型,与相应的对照组相比左侧精索静脉直径明显增大(P<0.01)。光学显微镜下观察睾丸组织,发现大鼠睾丸生精上皮退变,曲细精管萎缩,间质水肿和精子发育阻滞。EV组双侧睾丸的SOD活性显著低于相应的对照组(P<0.01),左侧睾丸比右侧睾丸更低,但无明显统计学意义(P>0.05)。EV组双侧睾丸间质细胞中Bcl-2的染色指数与相应的对照组相比均显著降低(P<0.01),左侧睾丸染色指数比右侧睾丸下降更明显(P<0.01),EV12周组与 EV8周组相比,EV12周组染色指数更低(P<0.05)。SOD活性与Bcl-2的染色指数在0.01水平有显著相...
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| 关 键 词： | 精索静脉曲张 氧化应激 凋亡 超氧化物歧化酶 Bcl-2 |
The Variation of SOD and Bcl-2 in the Testes of Experimental Varicocele Rats |
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| JIA Yue-feng,WANG Xin-sheng,MA Zheng,WANG Pei-tao. The Variation of SOD and Bcl-2 in the Testes of Experimental Varicocele Rats[J]. Progress in Modern Biomedicine, 2010, 10(4) |
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| Authors: | JIA Yue-feng WANG Xin-sheng MA Zheng WANG Pei-tao |
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| Abstract: | Objective: To establish experimental varicocele (EV) model in rats to measure the superoxide dismutase (SOD) activity and the Bcl-2 expression. Methods: The varicocele model was built by partial ligation of the left renal vein in adolescent male Wistar rats. 40 male adolescent Wistar rats were randomly divided into 8-week EV group, 12-week EV group and the corresponding sham-operated control group. The rats were killed 8 weeks and 12 weeks after operation, and the activity of SOD was determined by Colorimet... |
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| Keywords: | Varicocele Oxidative Stress Apoptosis SOD Bcl-2 |
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