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Cholinergic innervation of the retrosplenial cortex via the fornix pathway as determined by high affinity choline uptake,choline acetyltransferase activity,and muscarinic receptor binding in the rat
Authors:Sandra L Gage  Stanley R Keim  Jay R Simon  Walter C Low
Institution:(1) Program in Medical Neurobiology, Indiana University School of Medicine, Indianapolis, Indiana;(2) Dept of Anatomy, Indiana University School of Medicine, Indianapolis, Indiana;(3) Dept of Psychiatry, Indiana University School of Medicine, Indianapolis, Indiana;(4) Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, Indiana;(5) Dept of Neurosurgery, University of Minnesota Medical School Lions Research Building Room 421, 2001 Sixth Street SE, 55455 Minneapolis, Minnesota;(6) Dept of Physiology, University of Minnesota Medical School, Minneapolis, Minnesota;(7) Program in Neuroscience, University of Minnesota Medical School, Minneapolis, Minnesota
Abstract:The cholinergic projections from basal forebrain nuclei to the retrosplenial cortex (RSC) have previously been studied using a variety of histological approaches. Studies using acetylcholinesterase (AChE) histochemistry and choline acetyltransferase (ChAT) immunocytochemistry have demonstrated that this projection travels via the cingulum on route to the RSC. Preliminary studies from our laboratory, however, have shown that the fornix may also be involved in this projection. The present study uses the combination of pathway lesions, and the analysis of cholinergic neurochemical markers in the RSC to determine the role of the fornix in the cholinergic projection to the RSC. High affinity choline uptake (HACU) and ChAT activity were measured in the RSC of control rats, animals with cingulate lesions, and animals with fornix plus cingulate lesions. Fornix plus cingulate lesions resulted in significant deceases in HACU and ChAT activity in comparison to cingulate lesions alone. Muscarinic receptor binding was also evaluated in combination with the various lesions, and a significant increase in retrosplenial receptor binding was noted following fornix lesions. Together, these results support the concept of a fornix-mediated cholinergic pathway to the RSC.
Keywords:Retrosplenial cortex  fornix  cholinergic fibers  high affinity choline uptake  choline acetyltransferase  basal forebrain
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