In situ dark field microscopy for on-line monitoring of yeast cultures |
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| Authors: | Ning Wei Jia You Karl Friehs Erwin Flaschel Tim Wilhelm Nattkemper |
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| Institution: | (1) Bielefeld University, Faculty of Technology, Fermentation Engineering, Bielefeld, 33594, Germany;(2) Bielefeld University, Faculty of Technology, Applied Neuroinformatics Group, P.O. Box 100131, Bielefeld, 33501, Germany |
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| Abstract: | A new-type in situ probe has been developed to acquire dark field images of yeast in bioreactors. It has been derived from
an in situ bright field microscope that is able to measure cell density in bioreactors during fermentation processes. The
illumination part of the probe has been replaced with a dark field device, in which an aspheric condenser is used, so that
high contrast dark field images can be obtained. The technique of second imaging is implemented to improve the sharpness of
the images by means of a relay lens. This new in situ probe is expected to enable the evaluation of the cell viability without
staining owing to modern image processing. |
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| Keywords: | Dark field microscopy Aspheric lens Fermentation Yeast Cell density Biomass Viability |
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