Immunofluorescent localization of ovine placental lactogen |
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| Authors: | J Martal J Djiane Dr M P Dubois |
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| Institution: | (1) I.N.R.A., Physiologie de la Lactation, Jouy-en-Josas, France;(2) I.N.R.A., Physiologie de la Reproduction, Nouzilly, France;(3) Institut National de la Recherche Agronomique, Laboratoire de Neuroendocrinologie Sexuelle, C.R.V.Z., Nouzilly, Boite Postale no 1, 37380 Monnaie, France |
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| Abstract: | Summary The localization of ovine placental lactogen (OPL) was studied using the immunofluorescence method. The placentome sections were treated by an indirect technique with anti-OPL antibodies obtained from rabbits injected with purified hormone. OPL was located in large cells of the monostratified epithelium of chorionic villi. These cells are mono- or binucleated and PAS-positive. The immunological reaction was inhibited by the specific antigen (OPL, 400 g/ml undiluted anti-OPL antiserum) but not by ovine prolactin, bovine growth hormone, human placental lactogen nor by any other polypeptidic hormone tested. Reciprocally, the localization of OPL-secreting cells was unsuccessful with antibodies raised against these control hormones. |
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| Keywords: | Placenta sheep Ovine chorionic somato-mammotrophin Ovine placental lactogen Immunofluorescence |
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