Investigation on the biodegradability of mycotoxins nivalenol (NIV) and deoxynivalenol (DON) in a rusitec fermentor and their monitoring by HPLC/MS |
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Authors: | E Razzazi J Böhm KE Ahmed B Cecon B Rabus |
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Institution: | 1. Universit?t Wien, Institut für Ern?hrung / Veterin?rmedizinische, Veterin?rplatz 1 / A-1210, Wien
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Abstract: | Biodegradation of two B-trichothecenes nivalenol (NIV) and deoxynivalenol (DON) have been studied in a RUSITEC (rumen simulation
technique) system. The fermentation studies were carried out in vessels containing the rumen fluid. To reach steady state
an adaptation period of one week was carried out. The mycotoxin standards were then added into the fermentor in a concentration
of 1ppm NIV and 2ppm DON. The kinetics of NIV and DON biodegradability during the fermentation process were monitored by using
a rapid HPLC method combined with a mass spectrometer and an atmospheric pressure chemical ionisation (APCI-) interface. Also the effect of mycotoxin addition on different parameters such as ammonia production, pH, gas production
and volatile fatty acids have been studied. |
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