Gerbera jamesonii, a New Host of Fusarium oxysporum f.sp. tracheiphilum |
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Authors: | Marco Troisi Maria Lodovica Gullino Angelo Garibaldi |
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Affiliation: | Authors' address: Centre of Competence for the Innovation in the Agro-Enviromental Sector (AGROINNOVA), Universitàdegli Studi di Torino, via L. da Vinci 44, 10095 Grugliasco, Italy (correspondence to Marco Troisi. E-mail: ) |
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Abstract: | The random amplified polymorphic DNA (RAPD) technique was used to analyze the total genomic DNA of pathogenic isolates of Fusarium oxysporum on Gerbera jamesonii by comparing them to representatives of the formae speciales chrysanthemi and tracheiphilum. A close genetic relationship was observed among most of the new isolates from G. jamesonii. They shared RAPD markers with the tested representatives of the forma specialis chrysanthemi . Some isolates of those tested from diseased G. jamesonii were placed in a different cluster, which included representative isolates of forma specialis tracheiphilum . This is the first report of F. oxysporum f.sp. tracheiphilum on G. jamesonii. A rapid protocol for DNA extraction directly from fungal colonies grown on potato dextrose agar allowed complete analysis in less than 4 h. |
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Keywords: | RAPD Fusarium wilt Compositae physiological races Italy |
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