The Effect of Bacillus subtilis on in vitro Growth and Pathogenicity of Agrobacterium tumefaciens |
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| Authors: | F M Hassanein M A El-Goorani |
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| Institution: | Sabahia Research Station, Institute of Plant Pathology, Agricultural Research Center, Bachous, Alexandria (Egypt);Department of Plant Pathology, Faculty of Agriculture, University of Alexandria, Alexandria (Egypt). |
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| Abstract: | Five isolates of Bacillus subtilis isolated from the soil, were found to be antagonistic to 6 isolates of Agrobacterium tumefaciens in vitro. Inoculation of B. subtilis in wounded castor bean plants 30 min before or simultaneous inoculation with A. tumefaciens resulted in excellent control of the crown gall symptoms on the host within 50 days of inoculation. Application of B. subtilis 30 min after inoculation with A. tumefaciens did not result in appreciable disease reduction. Treatment of the tested plants by B. subtilis did not induce any phytotoxic injury or growth retarding side effects. It appears that B. subtilis could potentially be incorporated for crown gall control. However, further tests are needed to test this biological control agent with other plant species especially fruits, nuts and vine nursery stock. |
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