Identification of the K99 (F5) fimbrial adhesin in commercial vaccines used against calf enteritis |
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| Authors: | C J Thorns J A Sawtell J A Miller G W Wood |
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| Institution: | Ministry of Agriculture, Fisheries and Food, Central Veterinary Laboratory, Weybridge, Surrey, U.K. |
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| Abstract: | A monoclonal antibody (Mab)-based antigen capture enzyme-linked immunosorbent assay (ELISA) was developed and standardised for the detection of the K99 adhesin of Escherichia coli in aqueous vaccines. A repeating epitope on the K99 antigen is first captured then detected using peroxidase-labelled antibody. The assay gave positive results with all the vaccines tested that were known to contain K99 and was specific, rapid and reproducible. |
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