Expression of Chromosome-integrated Hydrogen-uptake Genes in Cicer-Rhizobium |
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| Authors: | M Kunnimalaiyaan M L Lodha |
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| Institution: | 1. Genetic Engineering Unit, Centre for Biotechnology, JNU, New Delhi, 110067, India
2. Division of Biochemistry, Indian Agricultural Research Institute, New Delhi, 11 0012, India
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| Abstract: | Integration of H2-uptake (hup) gene cosmid pHU52 into the chromosomal DNA, conferred H2- uptake activity on the Hup- Cicer-Rhizobium strain G36–84 in the free-living state and in nodules. In five transconjugants (G36–84:: Tn5:: pHU52) derepressed for hup gene expression, the specific Hup activity ranged from 158 to 256 nmole H2 hr-1 mg-1 protein which was 42 to 64% lower than the activity obtained in transconjugant with pHU52 as an episome. Integration of the cosmid significantly improved the relative efficiency of symbiotic N2-fixation by imparting H2-recycling capability to Hup- Cicer-Rhizobium. Demonstration of Hup activity in the nodules of field grown chickpea plants suggests that the integrated hup genes are stably maintained in natural environment |
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