He—Ne激光辐照与UV-B辐射对小麦幼苗细胞器中Na^＋／K^＋-ATP酶活性的影响

分别采用5mJ·s^-1·mm^-2He-Ne激光辐照、10．08kJ·m·^-2,d^-1增强UV-B辐射及二者组合对小麦（Triticum aestivum）晋麦8号（Triticum aestivum‘Jinmai8’）幼苗进行处理。第5天开始测定各处理小麦幼苗叶片中线粒体、叶绿体及细胞溶质中Na^＋／K^＋-ATP酶活性的变化。结果表明，随着处理天数的增加，小麦幼苗叶片线粒体、叶绿体及细胞溶质中Na^＋／K^＋-ATP酶活性均在第6天下降，第7天升高，而后又逐渐下降。在处理的第7天，仅He—Ne激光辐照可使小麦幼苗叶片线粒体、叶绿体及细胞溶质中Na^＋／K^＋-ATP酶活性升高：增强UV-B辐射使各细胞器中Na^＋／K^＋-ATP酶活性下降：复合处理后小麦各细胞器中Na^＋／K^＋-ATP酶活性均高于UV-B单独辐射处理。实验结果表明，一定剂量的He-Ne激光辐照能够部分修复UV-B辐射对小麦幼苗细胞器中Na^＋／K^＋-ATP酶造成的损伤。

Effects of He-Ne Laser and UV-B Radiation on Na+/K+-ATPase Activity in Organelles of Wheat Seedlings

Seedlings of wheat （Triticum aestivum） were exposed to He-Ne laser irradiation with 5mJ·s^-1·mm^-2 power density, enhanced UV-B radiation （10.08 kJ·m·^-2,d^-1） and the combined treatment of He-Ne laser irradiation and enhanced UV-B radiation.From day 5, changes of Na^＋/K^＋-ATPase activity in mitochondria, chloroplasts and cellular solutes were measured to determine the effects of He-Ne laser irradiation and UV-B radiation on wheat.Na^＋/K^＋-ATPase activity in mitochondria, chloroplasts and cellular solutes of wheat seedlings was reduced at day 6, increased at day 7, then gradually decreased with treatment days. At day 7, He- Ne laser irradiation resulted in increased activity of Na^＋/K^＋-ATPase in mitochondria, chloroplasts and cellular solutes of wheat seedlings. The activity of Na^＋/K^＋-ATPase was reduced under UV-B radiation. The Na^＋/K^＋-ATPase activity in various organelles by combined treatments was greater than the activity with UV-B radiation. All these results suggested that damage to wheat seedlings induced by enhanced UV-B radiation in terms of activity of Na^＋/K^＋-ATPase in various organelles can be repaired in part by He-Ne laser irradiation.