Rosavin as a product of glycosylation by Rhodiola rosea (roseroot) cell cultures |
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Authors: | Furmanowa Miros?awa Hartwich Ma?gorzata Alfermann August W Ko?miński Wiktor Olejnik Marian |
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Institution: | 1.Dept. of Biology and Pharm. Bot., Medical University of Warsaw, ul. Banacha 1, 02-097, Warsaw, Poland ;2.Institut für Entwicklungs- und Molekularbiologie der Pflanzen, Heinrich-Heine Universit?t Düsseldorf, Universit?tsstr. 1, Geb. 26.13, 40225, Düsseldorf, Germany ;3.Dept. of Chemistry, Warsaw University, ul. Pasteura 1, 02-093, Warsaw, Poland ;4.Institute of Organic Chemistry, Polish Academy of Sciences, ul. Kasprzaka 44/52, 01-224, Warsaw, Poland ; |
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Abstract: | The paper discusses glycosylation of trans-cinnamyl alcohol to obtain the biologically active compound rosavin and possibly
other cinnamoylglycosides. Cell suspension cultures of Rhodiola rosea were established from callus of leaf origin cultured
under light in a modified Murashige and Skoog medium. Under these conditions, no rosavin was formed. However, when trans-cinnamyl
alcohol (2.5 mM; in MeOH) was added to the medium, after 72 h incubation cells transformed over 90% of the cinnamyl alcohol
into a number of unidentified products. The structure of potential rosavin accumulated in intracellular spaces was elucidated
as 3-phenyl-2-propenyl-O-(6'-O-α-L-arabinopyranosyl) -β-D-glucopyranoside] by means of chemical and spectral analysis using
TLC, HPLC, UV, LSIMS and NMR methods. Rosavin yields of 0.03–1.01% dry weight were obtained. The actual amount depended on
the cell strain cultured and the biotransformation period.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | biotransformation cinnamyl alcohol Crassulaceae glycosides in vitro culture isolation and structure elucidation precursor Sedum roseum |
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