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Synergism of cellulase enzymes in mixed culture solid substrate fermentation
Authors:Muhannad I Massadeh  Wan Mohtar Wan Yusoff  Othman Omar and Jalil Kader
Institution:(1) School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia
Abstract:Sugar cane bagasse was subjected to a mixed culture, solid substrate fermentation with Trichoderma reesei QM9414 and Aspergillus terreus SUK-1 to produce cellulase and reducing sugars. The highest cellulase activity and reducing sugar amount were obtained in mixed culture. The percentage of substrate degradation achieved employing mixed culture was 26% compared to 50% using separate cultures of the two molds. This suggests that the synergism of enzymes in mixed culture solid substrate fermentation have lower synergism than in pure culture.
Keywords:Aspergillus terreus  mixed culture  solid substrate fermentation  Trichoderma reesei
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