Mechanism of enhancement of prochymosin renaturation by solubilization of inclusion bodies at alkaline pH |
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Authors: | ZHANG Zhizhou ZHANG Yuying YANG Kaiyu |
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Affiliation: | (1) Institute of Microbiology, Chinese Academy of Sciences, 100080 Beijing, China |
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Abstract: | The renaturation efficiency of recombinant prochymosin depends on not only the renaturation condi-tions but also the solubilization (denaturation) conditions. Compared with pH 8, solubilization of prochymosin-contain-ing inclusion bodies at pH 11 (8 mol/L urea) results in onefold increase of renaturation efficiency ( ~ 40% vs. ~ 20 % ). Alkaline pH facilitates the solubilization of inclusion bodies via the breakage of intermolecular disulfide bonds. Moreover, alkaline pH renders prochymosin molecules to be in a more reduced and more unfolded state which undergoes refolding readily. |
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Keywords: | solubilization of inclusion bodies renatnration of recombinant prochymosin. |
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