碳酸钙胁迫对枳壳愈伤组织生理特性的影响

石灰性土壤上富含碳酸钙是导致柑桔缺铁黄化的主要因素。碳酸钙胁迫对枳壳愈伤组织生理影响的研究结果表明除全Fe含量外,CaCO₃浓度与POD活性、SOD活性、CAT活性、活性Fe含量、可溶性蛋白质含量、愈伤组织增殖均呈显著或极显著负相关,与培养液pH值呈显著相关,随着培养天数的增加,POD活性、活性Fe含量、可溶性蛋白质含量、愈伤组织增殖呈降低趋势,SOD、CAT活性先升后降,全Fe含量在25日出现沉积,高浓度CaCO₃下培养液pH值仅在20日左右略有降低。

Responses of calluses of Poncirus Trifoliata to calcareous stress

Poncirus trifoliata, an important rootstock of citrus crops, suffered from chlorosis in CaCO3-rich soil,which is one of the difficult-to-tackle problems in the citrus industry in China. Experiments were conducted on the responses of calluses of Poncirus trifoliata to calcareous stress. Various stress levels were made by adding 0, 0.1% , 0.4%, 0.7%, and 1.0% CaCO3 in MT medium. The results showed that the whole CaCO3 stress caused poor ability of H exudation and low proliferation, weakened activity of peroxidese(POD) and decreased contents of active iron and soluble protein. CaCO3 stress enhaced the activities of superoxide dismutase(SOD)and catalase(CAT)during the initial culture period and weakened it afterwards.,and enriched total iron for 25 days culture. The negative correlation between CaCO3 concentration and the activities of POD, SOD, CAT, the content of active iron , the content of soluble protein and callus growth is signicant at the 0.01 or 0.05 level. The positive correlation between CaCO3 concentration and medium pH is signicant at the 0.01 level.