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Human granulocyte colony stimulating factor (hG-CSF): cloning,overexpression, purification and characterization
Authors:Ana LS Vanz  Gaby Renard  Mario S Palma  Jocelei M Chies  Sérgio L Dalmora  Luiz A Basso  Diógenes S Santos
Institution:1.Programa de Pós-Gradua??o em Biologia Celular e Molecular,Partenon,Brazil;2.Centro de Pesquisas em Biologia Molecular e Funcional, Instituto de Pesquisas Biomédicas,Partenon,Brazil;3.Quatro G Pesquisa e Desenvolvimento LTDA,Partenon,Brazil;4.Departamento de Biologia, Instituto de Biociências,Laboratório de Biologia Estrutural e Zooquímica, Centro de Estudos de Insetos Sociais, Universidade Estadual Paulista,Rio Claro,Brazil;5.Departmento de Farmácia Industrial,Centro de Ciências da Saúde, Universidade Federal de Santa Maria,Santa Maria,Brazil
Abstract:

Background  

Biopharmaceutical drugs are mainly recombinant proteins produced by biotechnological tools. The patents of many biopharmaceuticals have expired, and biosimilars are thus currently being developed. Human granulocyte colony stimulating factor (hG-CSF) is a hematopoietic cytokine that acts on cells of the neutrophil lineage causing proliferation and differentiation of committed precursor cells and activation of mature neutrophils. Recombinant hG-CSF has been produced in genetically engineered Escherichia coli (Filgrastim) and successfully used to treat cancer patients suffering from chemotherapy-induced neutropenia. Filgrastim is a 175 amino acid protein, containing an extra N-terminal methionine, which is needed for expression in E. coli. Here we describe a simple and low-cost process that is amenable to scaling-up for the production and purification of homogeneous and active recombinant hG-CSF expressed in E. coli cells.
Keywords:
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