Human granulocyte colony stimulating factor (hG-CSF): cloning,overexpression, purification and characterization |
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| Authors: | Ana LS Vanz Gaby Renard Mario S Palma Jocelei M Chies Sérgio L Dalmora Luiz A Basso Diógenes S Santos |
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| Institution: | 1.Programa de Pós-Gradua??o em Biologia Celular e Molecular,Partenon,Brazil;2.Centro de Pesquisas em Biologia Molecular e Funcional, Instituto de Pesquisas Biomédicas,Partenon,Brazil;3.Quatro G Pesquisa e Desenvolvimento LTDA,Partenon,Brazil;4.Departamento de Biologia, Instituto de Biociências,Laboratório de Biologia Estrutural e Zooquímica, Centro de Estudos de Insetos Sociais, Universidade Estadual Paulista,Rio Claro,Brazil;5.Departmento de Farmácia Industrial,Centro de Ciências da Saúde, Universidade Federal de Santa Maria,Santa Maria,Brazil |
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| Abstract: | Background Biopharmaceutical drugs are mainly recombinant proteins produced by biotechnological tools. The patents of many biopharmaceuticals
have expired, and biosimilars are thus currently being developed. Human granulocyte colony stimulating factor (hG-CSF) is
a hematopoietic cytokine that acts on cells of the neutrophil lineage causing proliferation and differentiation of committed
precursor cells and activation of mature neutrophils. Recombinant hG-CSF has been produced in genetically engineered Escherichia coli (Filgrastim) and successfully used to treat cancer patients suffering from chemotherapy-induced neutropenia. Filgrastim is
a 175 amino acid protein, containing an extra N-terminal methionine, which is needed for expression in E. coli. Here we describe a simple and low-cost process that is amenable to scaling-up for the production and purification of homogeneous
and active recombinant hG-CSF expressed in E. coli cells. |
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