| 血管平滑肌细胞增殖及其代谢活性的测定 |
| |
| 引用本文： | 洪华山 王一波 等. 血管平滑肌细胞增殖及其代谢活性的测定[J]. 细胞生物学杂志, 2001, 23(1): 36-39 |
| |
| 作者姓名： | 洪华山 王一波 等 |
| |
| 作者单位： | [1]北京医科大学第三医院，北京100083 [2]福建医科大学附属 |
| |
| 基金项目： | 福建省教委(99A063)及国家教委博士点基金资助课题(9724). |
| |
| 摘 要： | 本首先报道培养的新西兰大白兔的血管平滑肌细胞（SMC）能对WST－1进行还原代谢，并联合应用WST－1和BrdU ELISA测定同一标本的SMC的增殖及其代谢活性。结果表明：随着胎牛血清浓度的增加，SMC活细胞数目，WST－1和BrdU的OD值逐渐增多，说明WST－1和同一标本的WST－1和BrdU ELISA联合测定方法是检测SMC增殖的指标，能客观地反映SMC的DNA合成和代谢活性。
|
| 关 键 词： | 血管平滑性 细胞增殖 代谢活性 测定 动脉粥样硬化 |
| 修稿时间： | 2000-02-21 |
ASSAY OF PROLIFERATION AND METABOLIC OF VASCULAR SMOOTH MUSCLE CELLS |
| |
| HONG Hua Shan CHEN Fen Rong ZHU Ying Bao WANG Yi Bo LIN Lan JIANG Qiong. ASSAY OF PROLIFERATION AND METABOLIC OF VASCULAR SMOOTH MUSCLE CELLS[J]. Chinese Journal of Cell Biology, 2001, 23(1): 36-39 |
| |
| Authors: | HONG Hua Shan CHEN Fen Rong ZHU Ying Bao WANG Yi Bo LIN Lan JIANG Qiong |
| |
| Abstract: | WST-1 that could be metabolized by the cultured smooth muscle cells( VSMC)of New Zealand white rabbit was firstly reported in this paper. In the same sample, WST-1 and BrdU colorimetric ELISA were also used simultaneously to measure the DNA synthesis and metabolic activity of VSMC. The results showed the viable cell number, the OD of WST-1 and BrdU were increased respectively with the increasing concentration of fetal calf serum in VSMC. It demonstrates that WST-1. WST-1 and BrdU ELISA simultaneous measurement can reflect objectively the DNA synthesis and metabolic activity of VSMC and detect proliferation of the VSMC. |
| |
| Keywords: | Smooth Muscle Cell Proliferation Metabolism WST-1 BrdU ELISA |
| 本文献已被 CNKI 维普 等数据库收录！ |
