| A型产气荚膜梭菌α-毒素基因的克隆与核苷酸序列分析 |
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| 引用本文： | 赵志军,许崇波,曾瑾,邓光存,王玉炯. A型产气荚膜梭菌α-毒素基因的克隆与核苷酸序列分析[J]. 生物技术, 2003, 13(4): 1-2 |
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| 作者姓名： | 赵志军 许崇波 曾瑾 邓光存 王玉炯 |
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| 作者单位： | 宁夏大学生命科学学院,宁夏,银川,750021 |
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| 基金项目： | 宁夏自然科学基金资助项目("α毒素基因工程亚单位苗的研制",200206) |
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| 摘 要： | 利用聚合酶链式反应(PCR)技术,从A型产气莫膜梭菌染色体基因组中扩增了1．2kb的α毒素基因。通过T4 DNA连接酶,将纯化的PCR产物与载体pGEM-T连接,转化受至体菌JM109中,经NcoI/EcoRI和BamHI/EcoRI双酶切分析,证明重组质粒pXCPA02中含有A型产气荚膜棱菌α毒素全基因。经核苷酸序列分析,明确了克隆的α毒素基因在重组质粒中的连接向位且核苷酸序列是正确的。
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| 关 键 词： | A型产气荚膜梭菌 α-毒素 基因克隆 核苷酸序列分析 |
| 文章编号： | 1004-311X(2003)04-0001-02 |
| 修稿时间： | 2003-03-16 |
Cloning and Nucleotide Sequencing of alpha-toxin Gene of Clostridium perfringens typeA |
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| ZHAO Zhi-Jun,XU Chong-Bo,ZENG Jing,DENG Guang-cun,WANG Yu-Jiong. Cloning and Nucleotide Sequencing of alpha-toxin Gene of Clostridium perfringens typeA[J]. Biotechnology, 2003, 13(4): 1-2 |
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| Authors: | ZHAO Zhi-Jun XU Chong-Bo ZENG Jing DENG Guang-cun WANG Yu-Jiong |
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| Abstract: | Alpha-toxin gene was amplified from genomic DNA of Clostridium perfringens type A by polymerase chain reaction(PCR).PCR product was inserted into vector pGEM-T directively.The recombinant plasmid pXCPA02 was identified by restriction endonucleases Nco I/ Eco RI and Bam HI/ Eco RI analysis and nucleotide sequencing.The results showed that alpha-toxin gene fragment of 1.2kb has been cloned with a positive nucleotide sequence. |
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| Keywords: | Clostridium perfringens type A alpha-toxin gene gene cloning nucleotide sequence |
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