Discovery of selective aldo-keto reductase ligands--an on-bead assay strategy |
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Authors: | Dixon Seth Robins Lori Elling Robert A Liu Ruiwu Lam Kit S Wilson David K Kurth Mark J |
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Institution: | Department of Chemistry, University of California, Davis, 95616-5295, USA. |
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Abstract: | An enzyme labeling and screening strategy for the discovery of ligands selective in binding two structurally similar members of the aldo-keto reductase family of enzymes is reported. The resulting fluorescence microscope data obtained by screening a 74,088 member library led to the identification of selective ligands for aldose reductase (ALR2) and aldehyde reductase (ALR1). Resynthesis results validate the selectivity of these ligands. |
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