Exchange of colicin receptor capacity between strains of Escherichia coli sensitive or resistant to colicin K-K235 |
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Authors: | David P. Brunner Gary S. Graham Ronald W. Treick |
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Affiliation: | (1) Department of Biological Sciences, Illinois State University, 61761 Normal, Illinois, USA;(2) Department of Microbiology, Miami University, 45056 Oxford, Ohio, USA;(3) Present address: Hyland Diagnostics, Inc., 60073 Round Lake, Illinois |
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Abstract: | Phage and colicin-resistant mutants were derived from Escherichia coli K-12P678. Two classes of phage T6 and colicin K-resistant mutants (genotype tsx) were isolated. Tsx-2 mutants, which demonstrated mucoid growth and increased sensitivities to many antibiotics, became sensitive to colicin K when pretreated with ethylenediaminetetraacetate (EDTA), whereas Tsx-1 mutants did not. Reassociation of EDTA-released material partially restored resistance to colicin K for Tsx-2 mutants. When EDTA-released material from strain P678 was associated with either class of K-resistant mutant, an increase in colicin K sensitivity resulted. Observations suggest that colicin K can act on its target site once it penetrates the cell surface. In addition, results suggest that functional colicin K receptors can be transferred from sensitive to resistant strains, thus conferring colicin sensitivity.Non-standard Abbreviations SDS sodium dodecyl sulfate |
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Keywords: | Colicin K Phage T6 tsx mutants Outer membrane Reconstitution EDTA treatment Escherichia coli |
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