家蚕丝素蛋白轻链基因（fib-L）启动子序列的克隆及其活性分析

家蚕Bombyx mori丝素蛋白轻链（fibroin light-chain, fib-L）基因fib-L具有在后部丝腺组织专一性、高效性表达的特点。为了利用其启动子构建能够表达外源基因的丝腺生物工厂，本实验对fib-L启动子活性进行了研究。通过PCR法克隆了fib-L启动子元件，序列分析显示fib-L启动子由位于-33 ～ -25处的TATA盒元件和位于－128～－121处的特征性序列GTCAATTT共同组成。用fib-L启动子控制报告基因DsRed进行家蚕BmN细胞和蚕体内的瞬时表达研究，结果表明fib-L启动子可以驱动DsRed报告基因在BmN细胞和家蚕后部丝腺组织中瞬时表达。

Cloning and activity analysis of the promoter of fibroin-L gene from the silkworm, Bombyx mori

The fibroin light-chain gene (fib-L) of the silkworm Bombyx mori is tissue-specifically expressed in the posterior silk glands at high-level. To establish a silk gland bio-factory to produce foreign gene in B. mori under the control of the promoter of fibroin light-chain gene (fib-L), the activity of the promoter was investigated. The fib-L promoter obtained by PCR was cloned and sequenced. The sequence analysis showed that the promoter of fib-L was constituted of the TATA box (-33-35) and the characteristic sequence GTCAATTT(-128-121). The reporter gene DsRed driven by fibroin light-chain gene promoter was transiently expressed in the BmN cells and the posterior silk gland. The result showed that the fibroin light-chain gene promoter could drive the report gene transiently expressing both in the BmN cells and the posterior silk gland of silkworm B. mori.