Synthesis and characterization of branched phosphopeptides: Prototype consolidated ligands for SH(32) domains |
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Authors: | Qinghong Xu Jie Zheng David Cowburn George Barany |
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Affiliation: | (1) Department of Chemistry, University of Minnesota, 55455 Minneapolis, MN, U.S.A.;(2) The Rockefeller University, 10021 New York, NY, U.S.A. |
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Abstract: | Summary This paper details the solid-phase synthesis by N-9-fluorenylmethyloxycarbonyl (Fmoc) chemistry of a series of bivalent consolidated ligands, branched peptides with lengths of 22 to 25 residues. The target peptides were designed to, and in fact do, interact with greater specificity and higher affinity with the SH2 and SH3 domains of Abelson kinase in an SH(32) dual domain construct. Fmoc-O-phospho-l-tyrosine[Fmoc-Tyr(PO3H2)-OH] was used to introduce the required phosphotyrosine residues, and Fmoc-N-1-(4,4-dimethyl-2,6-dioxocyclohexylidene)ethyl-l-lysine [Fmoc-Lys(Dde)-OH] was used to introduce a branch point that allowed proper orientation of individual ligands. The resultant product peptides were characterized by amino acid analyses and electrospray mass spectra.This paper is based on a presentation given at the Symposium on Peptide Structure and Design as part of the 31st Annual ACS Western Regional Meeting held in San Diego, CA, USA, October 18–21, 1995. |
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Keywords: | Phosphotyrosine Dde N /content/k4432j2j79122318/xxlarge949.gif" alt=" epsi" align=" BASELINE" BORDER=" 0" >-amino protecting group Lysine branching Src homology domains Abelson kinase |
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