| Abstract: | The chicken cellular proto-oncogene c-src was cotransfected into normal Rat-1 cells with the mouse dhfr gene. Selection for amplification of dhfr sequences resulted in co-amplification of the chicken c-src gene. Cell clones expressing varying levels of c-src associated kinase activity were isolated, none of these had a transformed morphology. In contrast, expression of v-src in Rat-1 cells resulted in morphological transformation and the ability to grow in soft agar in an anchorage independent way. |
