An Escherichia coli mutant unable to support site-specific recombination of bacteriophage lambda |
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Authors: | A Kikuchi E Flamm R A Weisberg |
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Affiliation: | Laboratory of Molecular Genetics National Institute of Child Health and Human Development National Institutes of Health, Bethesda, MD 20205, U.S.A. |
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Abstract: | We report the isolation of mutations in, and the characterization of, an Escherichia coli gene, hip, that is required for site-specific recombination of phage lambda. hip mutants are recessive and are located near minute 20 on the linkage map. The gene product is not vital to bacterial growth, since deletion mutants are viable. The absence of hip product reduces lambda integration to barely detectable levels and also reduces prophage excision, but less drastically. Certain mutations in the lambda int gene partially restore integration and excision in hip- hosts. Homologous recombination promoted by recA does not require hip function. In addition to their defect in site-specific recombination, hip mutants are unable to support lytic growth of phage Mu or of certain lambda mutants. Their pleiotropic phenotype closely resembles that of himA mutants, but complementation, mapping and DNA sequencing show that hip and himA are different genes. |
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Keywords: | Author to whom reprint requests should be addressed at: Building 6. Room 408. NIH Bethesda MD 20205 U.S.A. |
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