Stimulation of androgenesis in white cabbage (Brassica oleracea var. capitata) anthers by low temperature and anther dissection |
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Authors: | Boris Osolnik Borut Bohanec Sibila Jelaska |
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Affiliation: | (1) Agronomy Department, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 61111 Ljubljana, Slovenia;(2) Department of Molecular Biology, Faculty of Science, University of Zagreb, Rooseveltov trg 6, 41000 Zagreb, R. Croatia |
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Abstract: | Anther culture was performed on two local cultivars, Ljubljansko and Varadinsko, and the F1 cv. Krautman (Bejo-Zaden). The effects on androgenesis of hot and cold temperature treatments and different dissections of anthers were evaluated. In contrast to cv. Krautman, cvs. Ljubljansko and Varadinsko produced more embryos after cold pretreatment of flower buds (4°C, 48 h) than after standard treatment (35°C, 24h). Simultaneous cutting of the anther tip and removal of the filament gave the best results in comparison to other tested dissections. Microscopical observations of sectioned anthers revealed enhanced embryo development near the cut ends of the anthers. Ploidy analysis revealed the presence of haploids among embryos resulting from cold treatment (4°C, 48 h), treatment at elevated temperature (35°C, 24 h), and among embryos resulting from dissections of anther tips. |
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Keywords: | androgenesis anther dissections cold pretreatment hot treatment white cabbage |
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