Effect of high concentrations of sucrose on the enzymatic activity of alpha-chymotrypsin |
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Authors: | Todorova R Nedev K |
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Institution: | Institute of Biophysics, Bulgarian Academy of Sciences, Sofia. todorova@obzor.bio21.bas.bg |
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Abstract: | The effect of the low-molecular-mass natural reagents in high concentrations is important for investigating enzymatic reactions in near "in vivo" conditions and for optimisation of biotechnology processes. A model system, including p-nitrophenyl acetate as substrate and alpha-chymotrypsin as proteolytic enzyme, has been used to study the effect of high concentrations of sucrose, both influencing the viscosity of the reaction medium and acting as a nucleophilic effector (activator) on the enzymatic reaction. A kinetic scheme at high concentrations of nucleophilic effectors (sucrose) has been proposed. |
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