Paclobutrazol inhibition of sterol biosynthesis in a cell suspension culture and evidence of an essential role for 24-ethylsterol in plant cell division |
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| Authors: | P A Haughan J R Lenton L J Goad |
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| Affiliation: | 1. Department of Biochemistry, University of Liverpool, P.O. Box 147, Liverpool, L69 3BX, U.K.;1. Department of Agricultural Sciences, University of Bristol, Long Ashton Research Station, Long Ashton, Bristol, BS18 9AF, U.K.;1. Université de Toulouse, UPS, IRSAMC, Laboratoire de Chimie et Physique Quantiques, 118, route de Narbonne, F-31062 Toulouse, France;2. CNRS (UMR 5626), F-31062 Toulouse, France;3. Université de Toulouse, INPT, Ecole d’Ingénieurs de Purpan, 75, voie du TOEC, BP 57611, F-31076 Toulouse Cedex 03, France;1. State Key Laboratory of High Field Laser Physics, Shanghai Institute of Optics & Fine Mechanics, Shanghai 201800, PR China;2. Sorbonne University, Faculty of Science and Engineering, UMR 7605, case 128, 4 Place Jussieu, F-75252 Paris Cedex 05, France;3. LULI, Ecole Polytechnique, CEA, CNRS, Laboratoire pour l''Utilisation des Lasers Intenses, Physique Atomique dans les Plasmas Denses, F-91128 Palaiseau, France;4. Moscow Institute of Physics and Technology – MIPT, Institutskii per. 9, Dolgoprudnyi 141700, Russia;5. National Research Nuclear University MEPhI (Moscow Engineering Physics Institute), 115409 Moscow, Kashirskoe sh. 31, Russia;1. SSICA - Stazione Sperimentale per l’Industria delle Conserve Alimentari, V. le F. Tanara, 31/A, 43121, Parma, Italy;2. Lenz Instruments S.L., c/Veneçuela 31, Nave E, 08019, Barcelona, Spain;1. The Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, Midreshet Ben-Gurion, 84990, Israel;2. Division of Biological Sciences, Interdisciplinary Plant Group, Christopher S. Bond Life Sciences Center, University of Missouri, Columbia, MO, 65201, USA;1. Department of Agriculture & Fisheries, Crop & Food Science, Ayr Research Station, Ayr, Qld, 4807, Australia;2. CSIRO Agriculture & Food, Townsville, Qld, 4814, Australia;3. Department of Agriculture & Fisheries, Crop & Food Science, Leslie Research Facility, Toowoomba, Qld, 4350, Australia |
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| Abstract: | Growth of a celery (Apium gravidens) cell suspension culture was inhibited by the synthetic plant growth regulator paclobutrazol. Paclobutrazol caused a reduction in the incorporation of [2-14C]acetate into the 4-demethyl sterols (campesterol, sitosterol, stigmasterol) but radioactivity accumulated in the 4 alpha-methylsterols. The accumulating 4 alpha-methylsterols were identified as obtusifoliol and cycloeucalenol indicating that paclobutrazol was inhibiting sterol biosynthesis by blocking 14 alpha-demethylation. The inhibition of celery cell growth by paclobutrazol could be partially overcome by addition of cholesterol to the culture medium. However, addition of stigmasterol restored growth to the control value suggesting an essential role for a 24-ethylsterol to support plant cell division. |
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