Uninterrupted DNA synthesis during S-phase in untransformed diploid hamster fibroblasts |
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Authors: | A H Cawood J R K Savage |
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Institution: | (1) M.R.C. Radiobiology Unit, OX11 ORD Harwell, Didcot, Oxon, England;(2) Present address: Department of Physiology and Biochemistry, University of Reading, Whiteknights, Reading, Berks., England |
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Abstract: | Untransformed Syrian hamster fibroblasts in exponential growth were exposed to a pulse of 3H]-thymidine for 5 min, followed immediately by bromodeoxyuridine, and serial samples were taken up to 16 h. Preparations were autoradiographed and stained for replication banding. No cell with replication bands was found without significant 3H]-thymidine uptake, although the extent of uptake varied between sub-phases of S. Thus there is no indication of a total cessation of synthesis at any period during S-phase. |
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