Potassium channels in pituitary cells in the teleost,Gillichthys mirabilis |
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| Affiliation: | 1. State Key Laboratory of Cognitive Neuroscience and Learning, Beijing Normal University, Beijing, China;2. Department of Curriculum and Instruction, Northern Illinois University, DeKalb, IL, USA;3. School of Public Affairs and Administration, University of Electronic Science and Technology of China, Chengdu, China;4. Tianxing Institute of Education and Philosophy, Beijing Normal University, Beijing, China;1. School of Education, Zhaoqing University, PR China;2. Department of Human Science and Public Education, Zhuhai Campus of Zunyi Medical University, PR China;3. Jiangsu Key Laboratory of Mental Health and Cognitive Science, School of Psychology, Nanjing Normal University, PR China;1. The Chinese University of Hong Kong, Department of Social Work, Hong Kong;2. University of Kentucky, College of Social Work, United States;3. The University of Hong Kong, Department of Social Work and Social Administration, Hong Kong;1. College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, PR China;2. Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou 310014, PR China;1. Municipality of Kirkkonummi, Virkatie 1, FI-02400 Kirkkonummi, Finland;2. City of Helsinki, Department of Social Services and Health Care, Toinen linja 4 A, P.O. Box 6000, FI-00099 City of Helsinki, Finland;3. Finanssivalvonta, FIN-FSA, Snellmanninkatu 6, 00101 Helsinki, Finland;4. University of Eastern Finland, Department of Nursing Science, City of Helsinki, Department of Social Services and Health Care, P.O. Box 1627, FIN – 70211 Kuopio, Finland;1. Department of Regulatory Bioorganic Chemistry, The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki 567-0047, Japan;2. Chemical Research Laboratories, Central Pharmaceutical Research Institute, Japan Tobacco INC., 1-1, Murasaki-cho, Takatsuki, Osaka 569-1125, Japan |
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| Abstract: | We have examined whole-cell K+ currents and a Ca2+-dependent K+ channel at the single channel level in rostral pars distalis cells of Gillichthys mirabilis. Whole-cell K+ currents activated by depolarizing pulses have an inactivating component and a sustained component. The magnitude of both of these components is increased when a hyperpolarizing prepulse is delivered prior to depolarization. Both components are partially blocked by application of 5 mM TEA+. The Ca-dependent K+ channel, (K(Ca)), was sensitive to 2 mM TEA+ in outside-out patches (O/O) but not in inside-out patches (I/O). Channel open probability (P(o)) was dependent on membrane potential (Vm), with depolarization leading to an increase in P(o). Calcium on the cytoplasmic face of I/O patches increased channel P(o) in a dose-dependent manner. A portion of the single K(Ca) channels studied displayed inactivation after depolarizing pulses. These channels may be a component of the inactivating whole-cell current. |
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