Isolation and sequencing of the cDNA encoding phosphatidylinositol transfer protein from rabbit lung |
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| Institution: | 1. Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia, Canada;2. Centre for Cell Biology, Development, and Disease, Simon Fraser University, Burnaby, British Columbia, Canada;1. Department of Molecular & Cellular Medicine, Texas A&M Health Science Center, College Station, TX 77843, USA;2. Department of Biochemistry & Biophysics, Texas A&M University, College Station, TX 77843, USA;3. Department of Chemistry, Texas A&M University, College Station, TX 77843, USA |
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| Abstract: | The cDNA clones encoding rabbit lung phosphatidylinositol transfer protein (PI-TP) were isolated and sequenced. The putative polypeptide consisted of 270 amino acid (aa) residues, the same as human PI-TP, but one aa residue less than the PI-TP of rat and mouse. PI-TP RNA expression in various tissues of a pregnant rabbit was analyzed by Northern blot. Brain, placenta and fallopian tube had the highest PI-TP RNA expression. PI-TP RNA expression in alveolar epithelial type-II cells isolated from rabbit lung markedly increased after a 24-h culture, suggesting that PITP RNA expression in type-II cells can be modified by ambient factors. |
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